{"id":1476,"date":"2018-09-12T09:25:27","date_gmt":"2018-09-12T09:25:27","guid":{"rendered":"http:\/\/edren.org\/ren\/?page_id=1476"},"modified":"2024-02-09T13:42:04","modified_gmt":"2024-02-09T13:42:04","slug":"cross-match","status":"publish","type":"page","link":"https:\/\/edren.org\/ren\/handbook\/transplant-handbook\/cross-match\/","title":{"rendered":"Cross match"},"content":{"rendered":"<h3><span style=\"font-size: 24pt;\"><strong><span style=\"color: #993300;\">Virtual cross match (vXM)<\/span><\/strong><\/span><\/h3>\n<p>In a vXM, profiles of HLA antibody reactivity from recently tested recipient samples, plus HLA typing of a deceased donor, are used to predict reactivity in advance of donor cells becoming available. Enables transplant to go ahead with certainty and shorter cold ischaemic times. Only suitable for low-risk recipients with no detectable HLA-donor specific antibodies (DSA) or HLA antibody negative patients on the vXM list.<\/p>\n<h3><span style=\"font-size: 24pt;\"><strong><span style=\"color: #993300;\">Prospective cross match<\/span><\/strong><\/span><\/h3>\n<p>For all other recipients, a full crossmatch must be performed using donor lymphocytes with recipient serum. Recipient serum usually must be fresh; occasionally a recent stored sample may be useful but later confirmation will then usually be required.<\/p>\n<p>Send spleen and lymph nodes to SNBTS H&amp;I staff for an urgent crossmatch.\u00a0 Please ensure that a completed SNBTS histocompatibility \/ platelet immuno-haematology form is sent with the tissue.<\/p>\n<p>Results will be reported to the renal recipient coordinator. In addition, a direct conversation should be had between consultant surgeon on call and H&amp;I consultant.<\/p>\n<p style=\"text-align: center;\">&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;&#8230;.<\/p>\n<h3><span style=\"font-size: 24pt;\"><strong><span style=\"color: #993300;\">About crossmatching and methods to detect anti-donor antibodies (DSA)<\/span><\/strong><\/span><\/h3>\n<p>Flow cytometry \u2013 fresh donor lymphocytes are mixed with recipient serum and analysed by Flow Cytometry to detect recipient antibody binding to T and B lymphocytes. As well as identifying HLA antibodies the assay may also detect clinically non-relevant non-HLA antibodies. Interpretation of the Flow crossmatch data requires HLA antibody data from a fresh or recent patient sample.<\/p>\n<p>Solid phase assay (e.g. Luminex) \u2013 recipient\u2019s serum is tested against a panel of purified HLA molecules to detect the precise targets of any anti-HLA antibodies. This assay is conducted without knowledge of potential donors, in advance of transplantation, or for monitoring post-transplant. Having uniformly low titres of DSA to donor antigens without a history of previous exposure to foreign HLA antigens makes a positive crossmatch very unlikely \u2013 this is the basis of the Virtual Crossmatch (vXM).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Virtual cross match (vXM) In a vXM, profiles of HLA antibody reactivity from recently tested recipient samples, plus HLA typing of a deceased donor, are used to predict reactivity in advance of donor cells becoming available. Enables transplant to go ahead with certainty and shorter cold ischaemic times. Only suitable\u2026<\/p>\n<p> <a class=\"continue-reading-link\" href=\"https:\/\/edren.org\/ren\/handbook\/transplant-handbook\/cross-match\/\"><span>Continue reading<\/span><i class=\"crycon-right-dir\"><\/i><\/a> <\/p>\n","protected":false},"author":2,"featured_media":0,"parent":1453,"menu_order":159,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_lmt_disableupdate":"no","_lmt_disable":"","footnotes":""},"class_list":["post-1476","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/edren.org\/ren\/wp-json\/wp\/v2\/pages\/1476","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/edren.org\/ren\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/edren.org\/ren\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/edren.org\/ren\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/edren.org\/ren\/wp-json\/wp\/v2\/comments?post=1476"}],"version-history":[{"count":16,"href":"https:\/\/edren.org\/ren\/wp-json\/wp\/v2\/pages\/1476\/revisions"}],"predecessor-version":[{"id":6312,"href":"https:\/\/edren.org\/ren\/wp-json\/wp\/v2\/pages\/1476\/revisions\/6312"}],"up":[{"embeddable":true,"href":"https:\/\/edren.org\/ren\/wp-json\/wp\/v2\/pages\/1453"}],"wp:attachment":[{"href":"https:\/\/edren.org\/ren\/wp-json\/wp\/v2\/media?parent=1476"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}